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        首頁 > 技術中心 > EZBioscience代理——北京百奧創新科技有限公司

        EZBioscience代理——北京百奧創新科技有限公司

         更新時間:2023-03-07 點擊量:509

               EZBioscienceZScience Biotechnology Corporation Limited是美國的一個生物技術品牌,主要專注于新技術開發和制造用于分子生物學的智能工具。EZBioscience的科學家和工程師是蛋白質表達、純化、結晶、結構測定和化學合成方面的專家,在分子克隆、RNA純化和相關檢測領域也擁有高度復雜的技術體系。EZBioscience的使命是通過向世界各地的科學家提供設計更巧妙的試劑和試劑盒,幫助使生物和醫學研究更容易。EZBioscience對醫療研究人員的承諾是以優惠的價格提供優質的產品。EZBioscience,使研究更容易。

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        EZBioscience熱銷產品

        產品一:EZ-press Single Cell to cDNA Kit (B0011)

        產品介紹:The EZBioscience® EZ-press SINGLE Cell to cDNA Kit allows researchers to analyze the gene expression of single or very low cell numbers (1 ~ 1,000 cells) in a reliable, sensitive and easy way. Cells are first lysed by the unique Cell Lysis Buffer. Then cDNA is produced and pre-amplified using gene specific primers from the cell lysate in the same tube. Due to the pre-amplification following reverse transcription, extremely low concentration of RNA from a very small amount of cells can be detected by the subsequent qPCR.

        產品二:EZ-press RNA Purification Kit (B0004D)

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        產品介紹:The EZBioscience® EZ-press RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of sources, without the need for toxic substances such as phenol or chloroform. The kit can be used with cultured cells. Biological samples are first lysed and homogenized in a strong denaturant containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After homogenization, ethanol is added to the lysate, creating conditions that promote selective binding of RNA to the silica-based membrane. The sample is then applied to the Spin Column, where the total RNA binds to the membrane, then the membrane is treated with gDNA Remover to eliminate the DNA. Contaminants are efficiently washed away by subsequent step of washing. The high-quality total RNA is then eluted in Elution Buffer. The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, and so on.

        產品三:4× EZscript Reverse Transcription Mix II (with gDNA Remover) (EZB-RT2GQ)

        產品介紹:TheEZBioscience®4× EZscript Reverse Transcription Mix II (with gDNA Remover) is a new-generation fast reverse transcription kit with higher reverse transcription efficiency compared to previous generations.

        The kit is suitable for real-time RT-PCR (RT-qPCR) that contains a gDNA Remover which can effectively eliminate the contamination of genomic DNA (gDNA) or other double stranded DNA during the analysis of gene expression. To accurately analyze gene expression, it is necessary to detect cDNA in samples without contaminating DNA. To avoid amplification of gDNA, primers can be designed on different exons spanning introns. However, there may be cases where a suitable primer cannot be designed, as with a gene with a single exon or a gene without a long intron. Also, it may be difficult to avoid unexpected amplification from gDNA due to non-specific amplification or the existence of pseudo-genes. Moreover, some labs are heavily contaminated by the PCR products of previous tests. This kit offers a potent gDNA Remover that can eliminate double stranded DNA in RNA sample in 5 minutes at room temperature without loss of RNA. Then the first strand of cDNA is synthesized by adding the 4× EZscript RT Mix II. Reaction products are applicable to subsequent PCR, qPCR.

        The mix contains reverse transcriptase, RNase Inhibitor, optimized buffer system, dNTPs, and Oligo dT18 and Random Hexamer as primers. The reverse transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. This product is also very suitable for reverse transcription using plant RNA.

                  EZBioscience代理——北京百奧創新科技有限公司,提供EZBioscience全系列產品。EZBioscience華中代理,EZBioscience華北代理,EZBioscience華南代理,EZBioscience華東代理。北京百奧創新科技有限公司作為一家專注于免疫學、細胞生物學、分子生物學等領域核心試劑產品的研發、生產與銷售的高新企業。北京百奧創新科技有限公司是中關村高新技術企業,于2017年在北京成立。百奧創新依托于中科院的科研技術力量,以中科院資深科學家為核心,整合經驗豐富的商業團隊及管理團隊組建而成。百奧創新在產品自主研發的同時,也與全球多家生命科學試劑供應商建立良好的合作關系,基于“客戶第一"的核心理念,向全國10000+客戶提供200000+優質產品。百奧創新將以品質樹立品牌,以服務贏得口碑,致力于成長為重要的生物原料及生物化學試劑開發與成果轉化的開TUO者,并成為行業的優質供應商。



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